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KMID : 0613820080180030422
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Journal of Life Science
2008 Volume.18 No. 3 p.422 ~ p.425
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Activities of Recombinant MT1-MMP Expressed in PANC-1 Cells
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Kim Hye-Nan
Chung Hye-Shin
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Abstract
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Membrane-type 1 matrix metalloproteinase (MT1-MMP) is a membrane-associated zinc-dependent endoproteinase involved in extracellular matrix remodeling. MT1-MMP hydrolyzes ECM proteins like collagen and is involved in cancer cell migration and metastasis. Caveolins are integral membrane proteins and play a role in formation of caveolae, specialized membrane microdomains involved in clathrin-independent endocytosis. Recombinant MT1-MMP was transiently expressed in PANC-1 cells. Cells expressing recombinant MT1-MMP were able to hydrolyze collagen and migrate on collagen coated trans-well. Both subjacent collagen degradation and the cell migration conferred by recombinant MT1-MMP were inhibited by co-transfection of plasmids containing caveolin-1 cDNA. The results support that MT1-MMP is localized in lipid raft of the membrane and MT1-MMP activities in invasive cells could be inhibited by caveolin.
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KEYWORD
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MT1-MMP, caveolin, MMP-2 activation, collagen degradation, cell migration
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